beta-Catenin antibody (pAb)

Aliases: CTNNB, MRD19, armadillo
Catalog No: 61487 Format: 100 µl $435 Buy
Catalog No: 61488 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
90 kDa


Western Blot Validated Immunoprecipitation Validated

Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution


This antibody was raised against a peptide within the N-terminal region of human beta-Catenin.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


beta-Catenin antibody (pAb) tested by immunoprecipitation.

beta-Catenin antibody (pAb) tested by Immunoprecipitation.
10 µl of beta-Catenin antibody was used to immunoprecipitate beta-Catenin from 500 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the beta-Catenin antibody at a dilution of 1:500.

beta-Catenin antibody (pAb) tested by Western blot.

beta-Catenin antibody (pAb) tested by Western blot.
Detection of beta-Catenin antibody by Western blot analysis.
Lane 1: A549 nuclear extract (30 µg).
Lane 2: DU145 nuclear extract (20 µg).
Both probed with beta-Catenin antibody at a dilution of 1:500.


beta-Catenin is a key downstream component of the canonical Wnt signaling pathway, plays an important role in the regulation of cell adhesion and embryonic development. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of beta-Catenin via BTRC and subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activation of Wnt responsive genes.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting