NFATC2 antibody (pAb)

Aliases: NFAT1, NFATP
Catalog No: 61621 Format: 100 µl $435 Buy
Catalog No: 61622 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
120 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Immunogen

This antibody was raised against a peptide within the N-terminal region of human NFATC2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
NFATC2 antibody (pAb) tested by immunoprecipitation.

NFATC2 antibody (pAb) tested by Immunoprecipitation.
10 µl of NFATC2 antibody was used to immunoprecipitate NFATC2 from 500 µg of Raji nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the NFATC2 antibody at a dilution of 1:500.

NFATC2 antibody (pAb) tested by Western blot.

NFATC2 antibody (pAb) tested by Western blot.
Detection of NFATC2 by Western blot analysis. NFATC2 was detected using a 1:500 dilution of NFATC2 (pAb) with 30 µg of Ramos nuclear extract.

Background

NFATC2 (Nuclear Factor Of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting