Histone H3.1 / 3.2 antibody (mAb)

Clone: 1D4F2
Catalog No: 61629 Format: 100 µg $445 Buy
Catalog No: 61630 Format: 10 µg $105 Buy

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Antibody Type:
Protein A Chromatography
Molecular Weight:
17 kDa
Human, Other (Wide Range)


ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
ICC/IF: 0.4 - 2 µg/ml dilution
WB: 0.2 - 2 µg/ml dilution

Published Applications:
See references for more information. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against a peptide comprising amino acids 21-39 of human Histone H3.1. This region is 100% identical in human Histone H3.2.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


Histone H3.1 / 3.2 antibody (mAb) tested by immunofluorescence.

Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by immunofluorescence.
Top: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Bottom: Hoechst.

Histone H3.1 / 3.2 antibody (mAb) tested by Western blot.

Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by Western blot.
HeLa nuclear extract (20 µg) and recombinant human Histones (100 ng) were probed with Histone H3.1 / 3.2 antibody (mAb) at a 1 µg/ml dilution in lanes 1, 2, & 3. Histone H3 (mAb) (Catalog No. 61475) is also shown at a 0.25 µg/ml dilution in lanes 4 & 5.
Lane 1: Nuclear extract of untreated HeLa cells.
Lane 2: 100 ng recombinant human Histone H3.1 protein.
Lane 3: 100 ng recombinant human Histone H3.3 protein.
Lane 4: 100 ng recombinant human Histone H3.1 protein.
Lane 5: 100 ng recombinant human Histone H3.3 protein.


Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3.1 / 3.2 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3.1 / 3.2 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3.1 / 3.2 antibody (mAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting