STAT3 antibody (pAb)

RRID: AB_2793718
Catalog No: 61645 Format: 100 µl $435 Buy
Catalog No: 61646 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button

Antibody Type:
Affinity Purified
Molecular Weight:
96 kDa


Western Blot Validated

Application Notes

Validated Applications:
WB: 1:1,000 - 1:2,500

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against a peptide within the N-terminal region of human STAT3.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.



STAT3 antibody (pAb) tested by Western blot.
Detection of STAT3 by Western blot analysis. Nuclear extract of HepG2 cells (30 µg) probed with STAT3 antibody at a dilution of 1:2,000.


STATs (signal transducers and activators of transcription) are a family of 7 transcription factors that form part of the JAK-STAT signaling cascade. This cascade is the basis of the signal transduction mechanism for many cytokine receptors. Once activated by phosphorylation by JAKs, STATs translocate to the nucleus. Accumulation of STATs in the nucleus is both rapid and tightly controlled. A number of factors regulate the JAK-STAT pathway including STAT dephosphorylation by phosphatases, altered nuclear import-export dynamics of STAT, and STAT gene activation antagonists such as SOCS (suppressors of cytokine signaling) and PIAS (Protein Inhibitors of Activated STATs).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting