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Antibodies

Histone H3.3K27M antibody (pAb)

Catalog No: 61803 Format: 100 µl $425 Buy Now
Catalog No: 61804 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Reactivity:Human

Applications

Western Blot Validated

for Histone H3.3K27M antibody (pAb)Application Notes


Validated Applications:
Western Blot: 1 - 2 µg / ml

for Histone H3.3K27M antibody (pAb)Immunogen

This antibody was generated using a peptide containing a methionine substitution at lysine 27 of human Histone H3.

for Histone H3.3K27M antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

for Histone H3.3K27M antibody (pAb)References

 
Histone H3.3K27M antibody (pAb) tested by Western blot.

Western blot analysis of antibody specificity for Histone H3.3K27M
0.1 µg per lane of Recombinant Human Histone H3.3 Cat. No. 31295 (Lanes 1 & 3) or Recombinant Human Histone H3.3K27M Cat. No. 31552 (Lanes 2 & 4) were probed with AbFlex® Histone H3.3 antibody (rAb), Cat. No. 91191 at 2 µg/ml (Lanes 1 & 2) or Histone H3.3K27M antibody (pAb) at 0.05 µg/ml.  

Histone H3.3K27M antibody (pAb) tested by Western blot.

Western blot of Histone H3.3K27M pAb.
H1299 cell extracts transfected with human Histone H3.3 wild-type (wt) or various Histone H3.3 single amino acid substitutions were probed with Histone H3.3K27M antibody (pAb) at 2 µg/ml. Beta-actin was used as a loading control.

for Histone H3.3K27M antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription.Histone H3.3 point mutations (K27 and G34) are present in 1/3 of pediatric glioblastomas. Up to 78% of diffuse intrinsic pontine gliomas (DIPGs) carries K27M and 36% of non-brainstem gliomas carries either K27M or G34R/V mutations. H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation.

for Histone H3.3K27M antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3.3K27M antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3.3K27M antibody (pAb)
 

Data Thumbnails

Histone H3.3K27M antibody (pAb) tested by Western blot.

Western blot of Histone H3.3K27M pAb.
(Click image to enlarge and see details.)

Histone H3.3K27M antibody (pAb) tested by Western blot.

Western blot of Histone H3.3K27M pAb.
(Click image to enlarge and see details.)