Expressed In: Synthetic Protein Species: N/A
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Active Motif biotinylated histone peptides are suitable for use in a variety of binding, pull-down and enzymatic assays for the study of proteins that interact with histones and that read, write or erase histone modifications.
Spin vial briefly in microfuge. Reconstitute with sterile distilled water and vortex briefly until resuspended. Aliquot reconstituted peptide to avoid repeated freeze-thawing.
Reference: Fuchs, S.M. et. al. Curr Biol. 2011 Jan 11; 21(1): 53–58.
A synthetic peptide derived from Histone H3.1, amino acids 1-21, modified by methylation on lysine 9, with an added lysine residue containing a biotin moiety and a C-terminal amide group (CONH2). C-term biotinylated Lys residue is separated with two Gly (provide short spacer) and one Tyr (provides accurate UV quantification) from the rest of the peptide sequence. The presence of the lysine residue is for quantitation and does not affect peptide functionality.
Peptide Sequence: ARTKQTAR-Kme2-STGGKAPRKQLA - GGYK(Biotin)- NH2
Calculated MW Mavg 2913.43
Observed MW Mavg
Purity: 95% by HPLC and Mass Spectrometry. 2914.48
Purity: 95% by HPLC and Mass Spectrometry.
Store lyophilized peptide at -20°C. Peptides in solution are stable for 6 months at -80°C.