Recombinant NFκB3 (RELA / p65) protein

Catalog No: 81086 Format: 20 µg $405 Buy
Catalog No: 81786 Format: 1 mg $3,500 Buy
Expressed In: Baculovirus Protein Species: Human

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Recombinant NFκB3 (RELA / p65) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.


NFκB3 (Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3), also known as RELA (V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A) or p65, is a ubiquitous transcription factor involved in several biological processes. NFκB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.

The NFκB dimers bind at κ-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different κ-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NFκB is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NFκB complexes are held in the cytoplasm in an inactive state complexed with members of the NFκB inhibitor (Iκ-B) family. In a conventional activation pathway, Iκ-B is phosphorylated by Iκ-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NFκB complex which translocates to the nucleus. NFκB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NFκ-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of Iκ-B upon NFκB in the cytoplasm is exerted primarily through the interaction with p65. 

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human NFκB3 (RELA / p65) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_068810.3) with an N-terminal FLAG tag. The molecular weight of the protein is 61.3 kDa. 

Recombinant NFKB3 (RELA / p65) protein gel

Recombinant NFKB3 (RELA / p65) protein gel
10% SDS-PAGE Coomassie staining
MW: 61.3 kDa
Purity: >80%


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.