Recombinant Histone H2A/H2B dimer

Catalog No: 81167 Format: 100 µg $395 Buy
Catalog No: 81867 Format: 1 mg $2,600 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Histone H2A and H2B are the core components of the nucleosome. H2A and H2B assemble H2A/H2B dimer, and two H2A/H2B dimers combine with H3/K4 tetramer to form histone octamer. The histone octamer is wrapped around by 146 bp DNA to assemble a nucleosome, which is the basic structural unit of chromatin in vivo.

Application Notes

Recombinant Histone H2A/H2B Dimer is suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant histone H2A/H2B Dimer consists of full length human histones H2A (accession number NP_003503.1) and full length human histone H2B (accession number NP_003509.1). Histone H2A and H2B were expressed in E. coli cells. Recombinant Histone H2A/H2B Dimer was assembled by dialysis and purified by gel filtration. The molecular weight of the Histone H2A/H2B Dimer is 27.9 kDa.

 

Recombinant Histone H2A/H2B Dimer
13% SDS-PAGE Coomassie staining

MW:
H2A: 14.1 kDa
H2B: 13.8 kDa

Purity: ≥ 90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.