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Recombinant PKM2 protein
| Aliases: Pyruvate kinase isozymes M1/M2, pyruvate kinase muscle isozyme, pyruvate kinase type K, cytosolic thyroid hormone-binding protein, thyroid hormone-binding protein 1, opa-interacting protein |
| Catalog No: 81302 | Format: 50 µg | $450 | Buy |
| Catalog No: 81602 | Format: 1 mg | $3,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PKM (Pyruvate Kinase M2) is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Full length human PKM2 protein was expressed in E. coli cells (accession number NP_872270.1) with a C-terminal 6xHis Tag. The molecular weight of the protein is 60.1 kDa.
Recombinant PKM2 protein gel
10% SDS-PAGE with Coomassie blue staining
MW: 60.1 kDa
Purity: >95%
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
