Recombinant SARS-CoV-2 Spike protein, S1 (RBD aa319-589)

Catalog No: 81344 Format: 100 µg $410 Buy

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Expressed In: 293 cells Protein Species: Virus

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies.

Application Notes

This product was manufactured as described in Protein Details and has been validated for use in ELISA. All available data for the protein is shown.

Protein Details

Recombinant SARS-CoV-2 Spike protein was expressed in 293 cells and contains a C-terminal 6XHis-Tag with an observed molecular weight of 45 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 

Recombinant SARS-CoV-2 Spike protein, S1 (aa319-589) protein gel.
1 µg of SARS-CoV-2 Spike protein was run on a 4-12% SDS-PAGE gel, stained with Coomassie blue.
MW: 40 kDa
Purity: >90%

SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay
A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 µg/mL RBD, aa 319-589 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 µg/mL RBD, aa 319-589. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.