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Recombinant NTRK3 (454-825) protein
| Aliases: Neurotrophic Receptor Tyrosine Kinase 3 |
| Catalog No: 81383 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 81683 | Format: 1 mg | $5,400 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
NTRK3 (Neurotrophic Receptor Tyrosine Kinase 3) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant NTRK3 (454-825) protein that includes amino acids 454-825 of human NTRK3 protein (accession number NP_001362740.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 43.5 kDa.
References
Human
Recombinant NTRK3 (454-825) protein
10% SDS-PAGE with Coomassie staining
MW: 43.5 kDa
Purity: >90%
HTRF assay for NTRK3 (454-825) activity
1 µM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
HTRF assay for NTRK3 (454-825) activity
1 µM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 µl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 µM ATP for 1 hour. Then 10 µl detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
