Recombinant PRKCI protein

Aliases: Protein Kinase C lota, PKC lota
Catalog No: 81424 Format: 20 µg $480 Add to Cart
Catalog No: 81524 Format: 1 mg $4,150 Add to Cart

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCI (Protein Kinase C Iota), a calcium- and diacylglycerol-independent serine/threonine-protein kinase that plays a general protective role against apoptotic stimuli and is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. PRKCI is necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. In cultured neurons, PRKCI prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, PRKCI may function downstream of phosphatidylinositol 3-kinase (PI(3) K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. PRKCI can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway.

Application Notes

Recombinant PRKCI protein is suitable for use in in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant PRKCI protein was expressed in baculovirus expression system as the full-length protein (accession number NP_002731.4) with a N-terminal FLAG tag. The molecular weight of the protein is 69.5 kDa.

References

Human

 
Recombinant PRKCl; protein

Recombinant PRKCl; protein
10% SDS-PAGE Coomassie staining
MW: 69.5 kDa
Purity: ≥ 90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.