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Recombinant PARG protein
| Aliases: Poly(ADP-ribose) glycohydrolase, PARG, Mitochondrial Poly(ADP-Ribose) Glycohydrolase |
| Catalog No: 82012 | Format: 20 µg | $505 | Add to Cart |
| Catalog No: 82612 | Format: 1 mg | $4,150 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PARG (Poly (ADP-ribose) glycohydrolase) catalyzes the hydrolysis of glycosidic bonds of ADP-ribose polymers, producing monomeric ADP-ribose units. Thus, in conjunction with poly(ADP-ribose) polymerase (PARP), PARG activity regulates the extent of in vivo poly(ADP-ribosyl)ation. PARG acts both as an endo- and exoglycosidase, releasing poly (ADP-ribose) of different lengths as well as ADP-ribose monomers. Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant PARG protein that includes full length of human PARG protein (accession number NP_003622.2) was expressed in Sf9 cells and contains an C-terminal His and FLAG tag with a molecular weight of 113.63 kDa. The purity of the protein is ≥ 75% by SDS-PAGE.
Recombinant PARG protein gel.
PARG protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.
MW: 113.63 kDa
Purity: >75%
ELISA for PARG activity
25 μM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of PARG were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.
ELISA for PARG activity
25 μM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of PARG were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
