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Recombinant SAE1 / SAE2 Complex
| Catalog No: 82102 | Format: 50 µg | $480 | Add to Cart |
| Catalog No: 82702 | Format: 1 mg | $2,950 | Add to Cart |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
The SAE1 / SAE2 complex (SAE) is the essential heterodimeric SUMO-activating enzyme that initiates the SUMOylation cascade, a critical post-translational modification regulating diverse cellular processes. SAE catalyzes the ATP-dependent activation of SUMO proteins (SUMO-1, -2, -3) through a two-step reaction: first forming a highenergy SUMO-adenylate intermediate, then transferring activated SUMO to the catalytic cysteine of the E2 conjugating enzyme UBC9 via a thioester bond. This activation step is indispensable for subsequent SUMO conjugation to target proteins by E3 ligases. By enabling SUMO modification, the SAE1 / SAE2 complex governs nuclear transport, genome stability, transcriptional regulation, DNA repair, and stress responses. Dysregulation of SAE function disrupts SUMOylation homeostasis and is implicated in cancer progression (e.g., breast cancer metastasis), neurodegenerative diseases, and viral pathogenesis, making it a potential therapeutic target. Its activity is dynamically regulated by cellular ATP levels and interaction partners like SENP proteases that reverse SUMOylation.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant SAE1 / SAE2 Complex that includes full length human SAE1 protein (accession number NP_005491.1) without any tag and full length human SAE2 protein (accession number NP_005490.1) with an N-terminal His tag was expressed in E. coli. The molecular weights of SAE1 and SAE2 are 39.83 kDa and 73.39 kDa, respectively.
Recombinant SAE1 / SAE2 complex gel
10% SDS-PAGE gel
MW:
SAE1: 28.3 kDa
SAE2: 73.39
Purity: >90%
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
