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Recombinant SRPK1 protein
| Aliases: SRSF protein kinase 1, SFRSK1 |
| Catalog No: 82108 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82708 | Format: 1 mg | $4,150 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
SRPK1 (SRSF protein kinase 1) is a serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant SRPK1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_003128.3) with an N-terminal FLAG tag. The molecular weight of the protein is 75.6 kDa.
Recombinant SRPK1 protein gel
10% SDS-PAGE gel
MW: 75.6
Purity: >95%
ADP-Glo assay for SRPK1 activity
0.1 µg/µl Myelin Basic protein was incubated with different concentrations of SRPK1 protein in a 5 µl
reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 µM DTT, 0.1 mg/ml BSA and 10 µM ATP at 30℃ for 1 hour, then 5 µl ADP-Glo Reagent was added. After 45 minutes incubation at RT, 10 µl Kinase Detection Reagent was added in the system. Following 45 minutes incubation at RT, luminescence was detected with a plate reader.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
