Cart
Products
Recombinant AHR / HIF1B Complex
| Aliases: AHR/ARNT |
| Catalog No: 82137 | Format: 10 µg | $480 | Add to Cart |
| Catalog No: 82737 | Format: 1 mg | $4,500 | Add to Cart |
Request a quote for a bulk order Request Quote
Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
The AHR / HIF1B complex (also termed AHR / ARNT) is a heterodimeric transcription factor that mediates cellular responses to environmental toxins, hypoxia, and metabolic cues. Upon ligand binding (e.g., dioxins or endogenous tryptophan metabolites), cytosolic AHR translocates to the nucleus and dimerizes with its constitutive partner HIF1B (HIF-1β/ARNT). This complex binds xenobiotic response elements (XREs), driving the expression of detoxification enzymes like CYP1A1. Beyond xenobiotic metabolism, it critically regulates immune cell differentiation, particularly in type 1 regulatory T cells (Tr1), where it orchestrates metabolic reprogramming: HIF1α (a HIF1B paralog) initiates early metabolic shifts, while AHR subsequently promotes HIF1α degradation to refine Tr1 cell fate. The complex also modulates macrophage polarization by suppressing glycolysis via the HIF-1α/GLUT1 axis, favoring anti-inflammatory M2 over pro-inflammatory M1 phenotypes. Dysregulation of AHR / HIF1B contributes to inflammatory diseases (e.g., rheumatoid arthritis) and cancer progression, highlighting its role in maintaining immune-metabolic homeostasis.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant AHR / HIF1B Complex that includes full length human AHR protein (accession number NP_001612.1) with an N-terminal FLAG tag and full length human HIF1B protein (accession number NP_001659.1) with an N-terminal His tag was expressed in Hi5 cells. The molecular weights of AHR and HIF1B are 97.42 kDa and 89.86 kDa, respectively.
References
Human
Recombinant AHR/HIF1B Complex protein gel.
AHR/HIF1B protein was run on an 10% SDS-PAGE gel and stained with Coomassie blue.
MW: AHR: 97.42 kDa,
MW: HIF1B: 89.96 kDa
Purity: > 80%
HTRF Assay for Recombinant AHR/HIF1B protein activity.
1 µM substrate dsDNA (5’ biotin-ATCGTGCGTGACAC 3’) was incubated with different concentrations of AHR / HIF1B Complex in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
