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Recombinant HES1 protein
| Aliases: Hairy and Enhancer of Split-1, HES1, HHL |
| Catalog No: 82153 | Format: 50 µg | $415 | Add to Cart |
| Catalog No: 82753 | Format: 1 mg | $2,950 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
Recombinant HES1 protein gel
4-20% SDS-PAGE gel
MW: 31.7 kDa
Purity: >60%
HTRF Assay for Recombinant HES1 activity.
1 µM substrate dsDNA (5’ biotin TTACACGAGCACGAGTTA 3’) was incubated with different concentrations of HES1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour . Anti-His antibody was used to detect reaction products.
Background
HES1 (Hairy and Enhancer of Split-1) is a key transcriptional repressor and a primary effector of the Notch signaling pathway. It plays a fundamental role in cell fate determination, tissue development, and stem cell maintenance by forming negative feedback loops that oscillate and drive cellular differentiation. Its dysregulated expression disrupts normal developmental programs and is critically involved in the pathogenesis of various cancers and neurological disorders.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
