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Recombinant RARA protein
| Aliases: Retinoic Acid Receptor Alpha |
| Catalog No: 82155 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82755 | Format: 1 mg | $4,150 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
Recombinant RARA protein gel
4-20% SDS-PAGE gel
MW: 52.04 kDa
Purity: >90%
HTRF Assay for Recombinant RARA activity.
1 µM substrate dsDNA (5’ biotin-CAGAGGTCATGGAAAGGTCATC 3’) was incubated with different concentrations of RARA protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour . Anti-FLAG antibody was used to detect reaction products HTRF assay was used for detection
HTRF Assay for Recombinant RARA activity.
1 µM substrate dsDNA (5’ biotin-CAGAGGTCATGGAAAGGTCATC 3’) was incubated with different concentrations of RARA protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour . Anti-FLAG antibody was used to detect reaction products HTRF assay was used for detection
Background
RARA (Retinoic Acid Receptor Alpha) is a ligand-dependent nuclear receptor and transcription factor that plays a central role in mediating the effects of retinoic acid, a critical derivative of vitamin A. It regulates gene networks essential for cell growth, differentiation, embryonic development, and apoptosis. As a master regulator of myeloid cell differentiation, its function is frequently disrupted in acute promyelocytic leukemia (APL) through chromosomal translocations, most commonly forming the PML-RARA oncoprotein. Therefore, RARA is not only a key developmental regulator but also a critical therapeutic target, with all-trans retinoic acid (ATRA) serving as a paradigm for targeted differentiation therapy in cancer.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
