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Recombinant NR1D1 protein
| Aliases: Nuclear Receptor Subfamily 1 Group D Member 1 |
| Catalog No: 82156 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82756 | Format: 1 mg | $4,150 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
Recombinant NR1D1 protein gel
4-20% SDS-PAGE gel
MW:68.08 kDa
Purity: >95%
HTRF Assay for Recombinant NR1D1
activity.
1 µM substrate dsDNA (5’ biotin CAGAGGTCAGTAGGTCAT 3’) was incubated with different concentrations of NR1D1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
Background
NR1D1 (Nuclear Receptor Subfamily 1 Group D Member 1), also known as Rev-Erbα, is a key transcriptional repressor that functions as a critical component of the mammalian circadian clock. It regulates the expression of core clock genes and integrates metabolic pathways, thereby orchestrating daily rhythms in glucose and lipid metabolism, inflammation, and cellular proliferation. Its dysfunction is implicated in circadian rhythm disorders, metabolic diseases, and certain cancers, highlighting its role as a pivotal link between circadian timing and physiological homeostasis.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
