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Recombinant MEN1 protein
| Aliases: Multiple Endocrine Neoplasia type 1 |
| Catalog No: 82157 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82757 | Format: 1 mg | $4,150 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
Recombinant MEN1 protein gel
4-20% SDS-PAGE gel
MW:69.72 kDa
Purity: >95%
HTRF assay for MEN1 activity/
3 μM H3K9me2 (1-21 aa) peptide was incubated with different concentrations of MEN1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
HTRF assay for MEN1 activity/
3 μM H3K9me2 (1-21 aa) peptide was incubated with different concentrations of MEN1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
Background
MEN1 (Multiple Endocrine Neoplasia Type 1) is a classic tumor suppressor gene encoding the nuclear protein menin. Menin acts as a critical molecular scaffold that regulates key cellular processes including transcription, genome stability, and cell division. It achieves this by orchestrating the assembly of large protein complexes, most notably with histone-modifying enzymes like the MLL/SET1 histone methyltransferase complex. Loss-of-function mutations in MEN1 disrupt these interactions, leading to dysregulated gene expression, uncontrolled cell proliferation, and ultimately the development of characteristic endocrine and non-endocrine tumors, defining the MEN syndrome.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
