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Recombinant RORC protein
| Aliases: RAR-related Orphan Receptor Gamma |
| Catalog No: 82160 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82760 | Format: 1 mg | $4,150 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
Recombinant RORC protein gel
4-20% SDS-PAGE gel
MW: 59.47 kDa
Purity: >95%
HTRF Assay for Recombinant RORC activity.
1 µM substrate dsDNA (5’ biotin-ATATAACTAGGTCATA
3’) was incubated with different concentrations of RORC protein in reaction buffer containing 50 mM HEPES NaOH pH 7.0, 100 µM ZnCl2, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
Background
RORC (RAR-related Orphan Receptor Gamma) , primarily known as the master transcription factor RORγt, is indispensable for the differentiation and function of immune cells, including Th17 cells and group 3 innate lymphoid cells (ILC3s). It directs the transcriptional program to produce pro-inflammatory cytokines such as IL-17A and IL-22, playing a central role in mucosal immunity, inflammation, and autoimmune pathogenesis. Consequently, RORC/RORγt is a prime therapeutic target for treating autoimmune and inflammatory diseases like psoriasis, multiple sclerosis, and inflammatory bowel disease.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
