Recombinant CRY1 protein

Aliases: Cryptochrome-1
Catalog No: 82172 Format: 20 µg $480 Add to Cart
Catalog No: 82772 Format: 1 mg $4,150 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

References

Human

 
Recombinant CRY1 protein

Recombinant CRY1 protein
4-20% SDS-PAGE gel with Coomassie blue staining
MW: 67.67 kDa kDa
Purity: >95%

Background

Recombinant human CRY1 (cryptochrome-1), also referred to by its gene symbol CRY1 or its historical name PHLL1 (photolyase-like 1), is a flavin adenine dinucleotide (FAD)-binding protein that functions as a core negative component of the circadian feedback loop by forming a repressor complex with PER proteins to inhibit CLOCK-BMAL1- mediated transcription. Beyond its central role in rhythm generation, this multifunctional protein is actively involved in ciliary function, intraflagellar transport, and the regulation of ciliary Hedgehog signaling, which is critical for embryonic development and tissue homeostasis. Furthermore, CRY1 plays an important role in mitochondrial function, where it translocates to mitochondria upon mitophagy induction to promote mitochondrial motility, clearance, and energymaintenance via AMPK signaling. The recombinant form, typically produced in expression systems such as E. coli or insect cells, retains these key biochemical properties and is widely used in research to study circadian mechanisms, metabolic pathways, and mitochondrial dynamics.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting