Recombinant MBD3 protein

Catalog No: 82177 Format: 20 µg $600 Add to Cart
Catalog No: 82777 Format: 1 mg $6,000 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant MBD3 protein gel

Recombinant MBD3 protein gel
4-20% SDS-PAGE gel
MW: 34.54 kDa
Purity: >90%

Background

Recombinant human MBD3 (methyl-CpG-binding domain protein 3), also known by its gene symbol MBD3 and alternative names including methyl-CpG-binding protein MBD3, is a core subunit of the nucleosome remodeling and deacetylase (NuRD) complex that plays a critical role in chromatin remodeling and transcriptional regulation. Unlike other family members, MBD3lacks the ability to bind methylated DNA due to amino acid substitutions in its methyl-CpG-binding domain, but instead contributes to NuRD complex integrity and function through protein-protein interactions that facilitate chromatin compaction and gene silencing. This recombinant form, typically produced in expression systems such as E. coli or insect cells, retains the key biochemical properties of the native protein, including its ability to interact with other NuRD complex components such as HDACs and CHD proteins to coordinate histone deacetylase activity and ATPdependent chromatin remodeling. MBD3is essential for embryonic development, stem cell pluripotency maintenance, and lineage commitment, functioning as a key regulator of gene expression programs by mediating NuRD complex recruitment to specific genomic loci and influencing diverse biological processes including hematopoiesis, neurogenesis, and cellular reprogramming.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting