Recombinant NAA15 / NAA10 Complex

Aliases: N-terminal acetyltransferase A complex
Catalog No: 82178 Format: 20 µg $480 Add to Cart
Catalog No: 82778 Format: 1 mg $4,130 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant NAA15/NAA10 protein gel

Recombinant NAA15/NAA10 protein gel
4-20% SDS-PAGE gel
MW: NAA15: 102.47 kDa
NAA10: 29.55 kDa
Purity: >95%

Background

Recombinant human NAA15 / NAA10 complex, also known as the NatA (N-terminal acetyltransferase A) complex, is a heterodimeric enzyme composed of the NAA15 auxiliary subunit (also called NARG1, NATH, GA19, or Tbdn100) and the NAA15 / NAA10 catalytic subunit (also known as ARD1, hARD1, or TE2). This recombinant complex, typically co-expressed in systems such as E. coli or insect cells, retains its native function as the major eukaryotic N-terminal acetyltransferase, catalyzing the transfer of an acetyl group from acetyl-CoA to the alpha-amino group of nascent polypeptides—a co-translational modification that is essential for protein stability, complex formation, and normal cellular function. Within the complex, NAA15 acts as a scaffold that binds to the ribosome and stabilizes the catalytic NAA10 subunit, while NAA10 performs the enzymatic acetylation of target proteins, including those involved in vascular, hematopoietic, and neuronal growth and development, and regulates key substrates such as HIF1A and TSC2 to influence cellular signaling and homeostasis.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting