Recombinant NSUN3 protein

Catalog No: 81183 Format: 20 µg $410 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

NSUN3 (NOP2/Sun RNA Methyltransferase Family Member 3), is a member of NSUN family. Gene Ontology (GO) annotations related to this gene include RNA binding and methyltransferase activity. NSUN3 is said to be a mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.

Application Notes

Recombinant NSUN3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant NSUN3 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_071355.1) with an N-terminal FLAG tag. The molecular weight of the protein is 39.5 kDa.

 

Recombinant NSUN3 protein
10% SDS-PAGE Coomassie staining
MW: 39.5 kDa
Purity: >80%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.