AbFlex® Myc-Tag antibody (rAb)

Catalog No: 91203 Format: 100 µg $385 Buy
Catalog No: 91204 Format: 10 µg $80 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 0.5-2 µg/ml dilution.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39279.

Immunogen

This antibody was raised against a peptide corresponding to amino acids 410-419 of human c-Myc (EQKLISEEDL).

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

References

 
AbFlex<sup>®</sup> Myc-Tag antibody (rAb) tested by Western blot.

AbFlex® Myc-Tag antibody (rAb) tested by Western Blot.
HEK293T cells were transiently transfected with a Myc-Tagged protein (Lane 1) or with empty vector (Lane 2).   Myc-Tagged protein is observed in lane 1 using this antibody.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Myc-Tag is a sequence derived from the human c-Myc protein that has been developed into an effective epitope for detection of tagged proteins expressed in vivo. 

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting