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Antibodies

AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb)

Catalog No: 91219 Format: 100 µg $375 Buy Now
Catalog No: 91220 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:210 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Enzyme-linked Immunosorbent Assay Validated

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)Application Notes


Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 µg/mL

Note: For optimal results, we recommend the addition of 0.1% Tween 20
to all Blotto solutions to reduce background. Individual optimization may
be required.

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 61383.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)Immunogen

This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)References

 
AbFlex<sup>®</sup> RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by Western blot.

AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb) (rAb) tested by Western Blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex® RNA Pol II CTD phospho Tyr1 antibody at 2 µg/ml.

AbFlex<sup>®</sup RNA pol II CTD phospho Tyr1 antibody (rAb) tested by Luminex bead-based specificity analysis.

AbFlex® RNA pol II CTD phospho Tyr1 antibody (rAb) tested by Luminex bead-based specificity analysis.
Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) antibody for RNA pol II CTD peptides. RNA pol II peptides were conjugated to MagPlex Luminex beads and incubated with 500 ng of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin (PE) and read in a Luminex instrument.
Luminex® is a registered trademark of Luminex Corporation.

AbFlex<sup>®</sup> RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by immunofluorescence.

AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by Immunofluorescence.
HeLa cells were stained with 2 µg /mL of AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) followed by anti-mouse IgG-488.

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb).

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by Western blot.

Western blot of AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup RNA pol II CTD phospho Tyr1 antibody (rAb) tested by Luminex bead-based specificity analysis.

AbFlex®
(Click to enlarge and view details).

AbFlex<sup>®</sup> RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by immunofluorescence.

Immunofluorescence stain of AbFlex® RNA Pol II CTD phospho Tyr1 antibody (rAb).
(Click image to enlarge and see details.)