SARS-CoV-2 Spike Antibody (AM004414)

Clone: AM004414 (414-4)
Aliases: COVID-19, Coronavirus-19
Catalog No: 91345 Format: 100 µg $445 Buy

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Antibody Type:
Recombinant
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Human
Molecular Weight:
141 kDa (full length S1 protein)
Reactivity:
Virus

Applications

Western Blot Validated Enzyme-linked Immunosorbent Assay Validated

Application Notes

Applications Validated by Active Motif:
ELISA
WB: 0.5 - 1 µg/ml

This antibody has been tested by ELISA and is specific for SARS-CoV-2 Spike protein S1 Subunit of Receptor Binding Domain (RBD).

Note: Clone AM004414, developed by Active Motif, was originally published as Clone 414-4. Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.

Published Applications

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

N/A

Buffer

140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.

 

SARS-CoV-2 Spike Antibody (clone AM004414) tested by ELISA.
SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

SARS-CoV-2 Spike Antibody (AM004414) tested by Western blot.
Lysates from HEK293 cells transfected (Lane 1 and 3) or untransfected (Lane 2) was probed with 0.5ug/mL of rAb (Lane 1 & 2) or an anti-flag Ab (Lane 3).
Lane 1: transfected flag-tagged SARS-CoV-2 S1 subunit
Lane 2 untransfected
Lane 3: transfected flag-tagged SARS-CoV-2 S1 subunit

Background

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies.

This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting