AbFlex® SARS-CoV-2 Spike Antibody (rAb) (AM043105)

Clone: AM043105 (105-43)
Catalog No: 91367 Format: 100 µg $385 Buy
Catalog No: 91368 Format: 10 µg $80 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
141 kDa (full length S1 protein)
Reactivity:
Virus

Applications

Enzyme-linked Immunosorbent Assay Validated

Application Notes

Applications Validated by Active Motif:
ELISA: 0.05 – 1 µg/ml

Immunogen

N/A - derived from COVID-19 patients who have cleared the virus. Their antibodies were screened for reactivity to SARS-CoV-2 and then sequenced and expressed as recombinant antibodies.

Buffer

140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.

 

AbFlex SARS-CoV-2 Spike Antibody (rAb) (AM043105) tested by ELISA using SARS Spike protein RBD.
SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 10 µg/mL and then incubated with a dilution series of AbFlex SARS-CoV-2 Spike Antibody (rAb) (clone AM009105). Bound antibodies were detected with anti-mouse IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

AbFlex SARS-CoV-2 Spike Antibody (rAb) (AM043105) tested by ELISA using SARS Spike protein ECD.
SARS-CoV-2 Spike Extracellular Domain (ECD a.a. 14-1213) protein was coated onto microtiter plates at 5 µg/mL and then incubated with a dilution series of AbFlex SARS-CoV-2 Spike Antibody (rAb) (clone AM009105). Bound antibodies were detected with anti-mouse IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® SARS-CoV-2 Spike (rAb) (AM002414) antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting