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AbFlex® PHD2 (EGLN1) antibody (rAb)
| Aliases: Egl-9 Family Hypoxia Inducible Factor 1, Hypoxia-Inducible Factor Prolyl Hydroxylase 2, HIF Prolyl Hydroxylase 2, HPH-2 |
| Catalog No: 91445 | Format: 100 µg | $400 | Add to Cart |
| Catalog No: 91446 | Format: 10 µg | $90 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
IP: 2 µg per IP
WB: 0.5 - 2 µg/ml dilution
IF: 2 µg per ml
Immunogen
This PHD2 antibody was raised against a peptide of human PHD2.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® PHD2 rAb antibody tested by Western blot.
Detection of PHD2 by Western blot analysis using 40 µg of HEK293 (Lane 1) and OSRC-2 (Lane 2) whole cell extracts and probed with PHD2 antibody at 0.5 µg/ml.
AbFlex® PHD2 rAb antibody tested by immunoprecipitation.
2 µg of antibody was used to immunoprecipitate PHD2 from 1 mg of HEK293F whole cell extract, 40% IP product (Lane 3) and 40 µg of Input proteins (Lane 1) were then run on SDS-PAGE and probed with the same antibody at 0.5 µg /ml. Tween-20 at 0.1% was added to blocking buffers to perform Western blot and reduce background. 2 µg of rabbit IgG was used as a negative control for IP (Lane 2).
AbFlex® PHD2 rAb antibody tested by immunofluorescence.
Staining of HeLa cells with PHD2 antibody at 2 µg/mL. Followed by Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L). DAPI at 594 nm.
Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® EGLN1 (PHD2) antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells. Prolyl Hydroxylase Domain-Containing Protein 2 (PHD2) or Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1) , also known as HIF-PH2 or HPH-2, is a prolyl hydroxylase. PHD2 is a cellular oxygen sensor that catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins under normoxic conditions. It can hydroxylate a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A, also HIF2A.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
