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AbFlex® TP53BP1 antibody (rAb)
| Aliases: Tumor protein p53 binding protein 1, p202, 53Bp1, TDRD30, p53BP1 |
| Catalog No: 91529 | Format: 100 µg | $400 | Add to Cart |
| Catalog No: 91530 | Format: 10 µg | $90 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.1 – 0.5 µg/mL
IF: 0.4 - 2 µg/mL
Immunogen
This TP53BP1 antibody was raised against a peptide of human TP53BP1.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® TP53BP1 rAb antibody tested by Western blot.
40 µg of HeLa (Lane1), K562 (Lane 2) and OSRC-2 (Lane 3) whole cell extracts were probed with AbFlex® TP53BP1 antibody at 0.1 µg/mL.
AbFlex® TP53BP1 rAb antibody tested by immunofluorescence.
TP53BP1 was detected in human HeLa cells using AbFlex TP53BP1 (rAb) at 2 µg/mL, followed by Multi-rAb CoraLite® Plus 594-Goat AntiRabbit Recombinant Secondary Antibody (H+L).
Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® TP53BP1 antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.
TP53BP1 (tumor protein p53 binding protein 1, also known as p202; 53BP1; TDRD30; p53BP1) is a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. It plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
