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NR3C1 antibody (rAb), 100 µg
| Clone: 2F22 |
| Aliases: NR3C1, GCR, GCCR, GR |
| Catalog No: 91611 | Format: 100 µg | $515 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:5000-1:50000 dilution
IHC: 1:175-1:700 dilution
Immunogen
This antibody was raised against the NR3C1 fusion protein.
Buffer
Purified IgG in PBS, pH 7.3, with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.
NR3C1 antibody (rAb) tested by Western blot.
Detection of NR3C1 by Western blot analysis using various lysates and probed with NR3C1 antibody at 1:10000 dilution.
NR3C1 antibody (rAb) tested by Immunohistochemistry. NR3C1 antibody was used in IHC to stain human prostate cancer tissue at a 1:350 dilution under 10X lens (Panel A) and under 40X lens (Panel B). Heat me antigen retrieval with Tris-EDTA buffer (pH 9.0).
Background
NR3C1 (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor)) is a receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
