Recombinant PXR protein

Aliases: NR1I2, BXR, PAR, PRR, SAR, SXR, ONR1, PAR1, PAR2, PARq
Catalog No: 31144 Format: 5 µg $430 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

5 µg of Recombinant PXR protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Please refer to product insert upon arrival for lot-specific concentration.

Application Notes

PXR can be used for Western blot (50 kDa), gel mobility shift assay, for protein-protein and small molecule-protein interaction assays. 1 ng is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 ng is sufficient for a protein-protein interaction assay detected by immuno-blot system. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Protein Details

Recombinant PXR (accession number NM_003889) is expressed in a baculovirus system; carries the full-length coding sequence and is purified by an affinity column in combination with FPLC chromatography.

References


This product was used in the following publications:
Wang, C., et. al. (2018). "Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity." Cell Death Dis. 9(8):819. PMID: 30050067. (Western Blot).

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.