Recombinant HDAC3 complex

Aliases: HD3; RPD3; RPD3-2
Catalog No: 31349 Format: 50 µg
Expressed In: Baculovirus Protein Species: Human

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Contents


This product has been discontinued:
Please refer to our alternative product Catalog No. 31526

50 µg recombinant HDAC3/SMRT complex supplied in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione and 20% glycerol.

Background

The HDAC3/SMRT subunits are members of the NCoR2 co-repressor complex that functions to mediate transcriptional silencing for various transcription factor pathways. HDAC3 is a Class I histone deacetylase with broad specificity. SMRT (also known as NCoR2) is a transcriptional coregulatory protein that binds nuclear receptors and aids in the recruitment of histone deacetylases to DNA promoter regions. Together, the HDAC3/SMRT complex functions to assist nuclear receptors in the downregulation of target gene expression.

Application Notes

The HDAC3/SMRT complex is suitable for use in histone deactylase assays. Specific Activity: 3000 pmol/min/μg. It can also be used to study enzyme kinetics, inhibitor screening, and selectivity profiling. Assay conditions: Prepare 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 20 μM HDAC substrate and HDAC3/SMRT complex. Incubate for 30 minutes at 37°C.

Protein Details

The Recombinant HDAC3 complex, active consists of the HDAC3/SMRT subunits of the NcoR2 corepressor complex. Recombinant full length HDAC3 (accession number NM_003883) was co-expressed in a baculovirus expression system with a C-terminal His-Tag (MW = 49.7 kDa) along with amino acids 395-489 of SMRT/NcoR2 (accession number NM_006312) containing an N-terminal GST-Tag (MW = 37.6 kDa).

 
Recombinant HDAC3 complex Coomassie gel

SDS-PAGE gel of Recombinant HDAC3/SMRT protein complex. Lane 1: 1 µg HDAC3/SMRT. Arrows indicate the HDAC3 and SMRT subunits. Lane 2: Protein Marker

Recombinant HDAC3 complex activity assay

HDAC3/SMRT activity assay.
Recombinant HDAC3/SMRT activity measured using a fluorescent histone deacetylase assay.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.