SMRT / NCoR2 antibody (mAb)

Clone: 5.10
Aliases: TRAC, CTG26, SMRTE, TRAC1, N-CoR2, TNRC14, TRAC-1, SMAP270, SMRTE-tau
Catalog No: 61105 Format: 100 µg $435 Buy
Catalog No: 61106 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
300 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Published Applications:
WB
See references for more information. Individual optimization may be required.

Immunogen

This SMRT / NCoR2 antibody was raised against a recombinant protein corresponding to amino acids 2004-2310 of human SMRT / NCoR2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

SMRT / NCoR2 (Nuclear receptor corepressor 2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, Thyroid and retinoic-acid-receptor-associated corepressor, TRAC) is a transcriptional corepressor for nuclear receptors. SMRT / NCoR2 has been shown to play a role in regulating immune response, mediating DNA repair, and in neuronal differentiation and cardiac development. SMRT / NCoR2 is part of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. It also includes TBL1, TBLR1, and GPS2 that help stabilize the overall structure of the complex. SMRT / NCoR2 mediates the transcriptional repression activity of nuclear receptors by promoting histone deacetylation and chromatin condensation, thus preventing access of the basal transcription machinery.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting