Recombinant HDAC5 protein, catalytic domain

Aliases: Histone Deacetylase 5, HD5; NY-CO-9
Catalog No: 31351 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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Contents


This product has been discontinued:
Please refer to our alternative product Catalog No. 31540

10 µg recombinant HDAC5 protein, catalytic domain, active supplied in a buffer of 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.

Background

HDAC5 (Histone Deacetylase 5) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Application Notes

Recombinant HDAC5 protein, catalytic domain is suitable for use in histone deacetylase (HDAC) assays. It can also be used to study enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: 2521 pmol/min/μg.

Protein Details

The catalytic domain, variant 1 (amino acids 657-1123) of human HDAC5 (accession number NM_005474), was expressed with a C-terminal His-Tag (MW = 51 kDa) using a baculovirus expression system.

 
HDAC5 protein Coomassie gel

HDAC5 catalytic domain protein gel.
HDAC5 catalytic domain run on an SDS-PAGE gel and stained with Coomassie blue.

HDAC5 activity assay

HDAC5 catalytic domain activity assay.
Recombinant HDAC5 catalytic domain activity measured using a fluorescent HDAC assay.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.