Recombinant HDAC5 protein

Aliases: HDAC-5, Histone Deacetylase 5, HD5
Catalog No: 31534 Format: 10 µg $420 Buy

Request a quote for a bulk order Request Quote


Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

HDAC5 (Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modi cation that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins.

Application Notes

Recombinant HDAC5 (656-1123) protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Protein Details

Recombinant human HDAC5 was expressed in a baculovirus expression system as the full length protein (accession number NP_001015053.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is 124 kDa. The purity of HDAC5 protein is >66% by SDS-PAGE.

 

Recombinant HDAC5 protein gel.
HDAC5 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

HDAC-Glo™ Class IIa Assay for HDAC5 (656-1122)activity
Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC5 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.

HDAC-Glo Class IIa Assay for HDAC5 activity
Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC5 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.