Sox2 antibody (pAb)

RRID: AB_2793356
Aliases: ANOP3, MCOPS3
Catalog No: 39823 Format: 100 µl $445 Buy
Catalog No: 39824 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
35 kDa
Reactivity:
Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:2,000 - 1:10,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IHC
ICC/IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Sox2 antibody was raised against a peptide corresponding to amino acids amino acids 111-140 mouse Sox2.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Sox2 antibody (pAb) tested by Western blot.
HeLa cell extract expressing mouse Sox2 (50 µg per lane) probed with Sox2 antibody at a dilution of 1:5,000.

Background

Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct-4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct-4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3.

qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting