Recombinant EHMT2 (G9a) protein

Aliases: EHMT2; BAT8; GAT8; NG36; KMT1C; C6orf30
Catalog No: 31410 Format: 20 µg $405 Buy
Catalog No: 31810 Format: 1 mg $3,500 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant EHMT2 (G9a) protein expressed in Sf9 cells and supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

Background

EHMT2 (G9a) is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2 (G9a) also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. EHMT2 (G9a) also weakly methylates Lys27 of histone H3 (H3K27me). In addition, EHMT2 (G9a) is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. EHMT2 (G9a) is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. EHMT2 (G9a) may also methylate histone H1. In addition to histone methyltransferase activity, EHMT2 (G9a) also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Application Notes

Recombinant EHMT2 (G9a) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

EHMT2 (G9a) is a histone methyltransferase that is specific to lysine 9 of histone H3 (H3K9). Methylation of H3K9 is a signal of transcriptional repression. Recombinant EHMT2 (G9a) protein (accession number NP_006700.3) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 134 kDa. The recombinant protein is >90% pure by SDS-PAGE.

References


This product was used in the following publications:
Mol. Cell. (2016). 61(2): 247-59. PMID: 26778125. (Histone Methyltransferase / HMT Assay)
Cell Death Dis. (2018). 9(10): 1038. PMID: 30305606.

 
EHMT2 (G9a) Coomassie gel

Recombinant EHMT2 (G9a) protein gel.
EHMT2 (G9a) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

HTRF Assay for EHMT2 (G9a) activity.

Recombinant EHMT2 (G9a) Activity Assay
3.3 µM H3K9me0 (1-21aa) peptide was incubated with EHMT2 (G9a) in reaction buffer for 3 hour at room temperature. EHMT2 (G9a) enzyme was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K9me2) was measured using H3K9me2-specific antibody.

Recombinant EHMT2 (G9a) protein

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.