Recombinant DNMT1 protein

Aliases: AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Catalog No: 31335 Format: 10 µg $435 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

10 µg recombinant DNMT1 protein supplied in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

DNMT1DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Application Notes

Recombinant DNMT1 is suitable for use in DNA methylation assays. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Active Motif has a collection of small-molecule epigenetic modulators that target DNMTs - click here to view the list.

Protein Details

Recombinant human DNMT1 was expressed in Sf9 cells via a baculovirus expression system as amino acids 2-1632 (accession number NM_001130823) with an N-terminal GST-Tag. The molecular weight of the protein is 211 kDa.

References


This product was used in the following publications:
J. Clin. Invest. (2018). 128(6):2376-2388. PMID: 29708513.

 

DNMT1 activity assay.
Recombinant DNMT1 activity was incubated with 400 µM S-adenosylmethionine (SAM) and DNA substrate, followed by detection with anti-5mC antibody and chemiluminescent detection.

DNMT1 protein gel.
DNMT1 (2.8 µg) run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue. Arrow indicates recombinant DNMT1.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.