Recombinant OGT protein

Aliases: O-linked N-Acetylglucosamine Transferase, GlcNAc, EC 2.4.1.186 HINCUT-1, O-GLCNAC, HRNT1
Catalog No: 31524 Format: 20 µg $410 Buy
Catalog No: 31924 Format: 1 mg $3,500 Buy
Expressed In: Baculovirus Protein Species: Mouse

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Contents

Recombinant full length Mouse OGT protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcetylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant mouse OGT was expressed in a baculovirus expression system as the full length protein (accession number NP_001277464.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is 118.7 kDa. The purity of OGT protein is >90 by SDS-PAGE.

 
Recombinant OGT protein gel.

Recombinant OGT protein gel. OGT protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.