Tet2 antibody (mAb)

Clone: 21F11
Aliases: MDS, KIAA1546
Catalog No: 61389 Format: 100 µg $435 Buy
Catalog No: 61390 Format: 10 µg $105 Buy
Catalog No: 61990 Format: 50 µg $250 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1k
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
220 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 1 - 2 µl per IP
WB: 1 - 2 µg/ml dilution

Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 1-300 of human Tet2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
Tet2 antibody (mAb) tested by immunoprecipitation.

Tes2 antibody (mAb) (Clone 21F11) tested by Immunoprecipitation.
Tet2 detection by Immunoprecipitation. Lane 1: Input (25 µg of HL-60 whole cell lysate). Lane 2: Negative control mAb. Lane 3: Tet2 antibody at 1 µg/ml. 250 µg of HL-60 whole cell lysate per IP.

Tet2 antibody (mAb) tested by Western blot.

Tes2 antibody (mAb) (Clone 21F11) tested by Western blot.
Tet2 detection by Western blot. Lane 1: HL-60 whole cell lysate (40 µg). Lane 2: Lymphoblastoid Tet2 negative (-/-) whole cell lysate (40 µg). Lane 3: Lymphoblastoid Tet2 positive (+/+) whole cell lysate (40 µg). All probed with Tet2 antibody at a 1 µg/ml dilution.

Background

Tet2 (tet methylcytosine dioxygenase 2) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation and differentiation in the case of inflammation by regulating gene expression and macrophage differentiation.

Positive Control

Active Motif's HL-60 nuclear extract (Catalog No. 36072) can be used as a positive control for Tet2 antibody.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Tet2 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting