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Recombinant Proteins & Enzymes

Recombinant SIRT4 (25-314) protein

Aliases: SIRT-4, Sirtuin 4, EC 3.5.1, SIR2L4, SIR2-Like 4
Catalog No: 31530 Format: 50 µg $405 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SIRT4 (25-314) proteinContents

Recombinant SIRT4 (25-314) protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SIRT4 (25-314) proteinBackground

SIRT4 (Sirtuin 4) is a member of the SIR2 / Sirtuin family of NAD+-dependant deacetylase enzymes. Sirtuin family enzymes can be found in the cytoplasm, mitochondria or nucleus of a cell and are ubiquitously expressed. SIRT4 has multiple enzymatic functions, such as NAD+-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. SIRT4 catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. It inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 catalyzes the transfer of ADP-ribosyl groups onto target proteins, such as mitochondrial GLUD1. It acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1. It also acts as a NAD+-dependent protein deacetylase to mediate deacetylation of Lys-471 of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis.

for Recombinant SIRT4 (25-314) proteinApplication Notes

Recombinant SIRT4 protein is useful for protein deacetylase, lipoamidase and ADP-ribosyl transferase assays and the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Active Motif has a collection of small-molecule epigenetic modulators that target SIRTs - click here to view the list.

for Recombinant SIRT4 (25-314) proteinProtein Details

Recombinant SIRT4 (25-314) protein that includes amino acids 25 -314 of human SIRT4 protein (accession number NM_012240.2) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 64 kDa. The purity of the protein is > 60% by SDS-PAGE.

Recombinant SIRT4 (25-314) SDS-PAGE gel.

Recombinant SIRT4 (25-314) 12.5% SDS-PAGE with Coomassie Blue staining
MW: 64 kDa

for Recombinant SIRT4 (25-314) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SIRT4 (25-314) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.


Technical Data Sheet

Recombinant SIRT4 (25-314) protein

Data Thumbnails

Recombinant SIRT4 (25-314) SDS-PAGE gel.

Recombinant SIRT4 Protein 12.5% SDS-PAGE with Coomassie Blue staining.
MW: 64 kDa
(Click image to enlarge and see details).