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Recombinant Proteins & Enzymes

Recombinant SIRT5 protein

Aliases: SIRT-5, Sirtuin 5, SIR2L5, SIR2-Like Protein 5
Catalog No: 31531 Format: 100 µg $405 Buy Now
Catalog No: 31931 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SIRT5 proteinContents

Full length recombinant SIRT5 protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SIRT5 proteinBackground

SIRT5 (Sirtuin 5) is a member of the SIR2 / Sirtuin family of NAD+-dependant deacetylase enzymes. Sirtuin family enzymes can be found in the cytoplasm, mitochondria or nucleus of a cell and are ubiquitously expressed. SIRT5 has multiple enzymatic functions, such as NAD+-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. SIRT5 catalyzes more efficiently removal of malonyl- and succinyl- and glutaryl- than acetyl-lysine modifications. SIRT5 activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting by mediating desuccinylation and deglutarylation of CPS1. SIRT5 also activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. It can modulate ketogenesis through the desuccinylation and activation of HMGCS2. SIRT5 can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

for Recombinant SIRT5 proteinApplication Notes

Recombinant SIRT5 protein is useful for protein deacetylase, demalonylase, desuccinylase and deglutarylase assays and the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Active Motif has a collection of small-molecule epigenetic modulators that target SIRTs - click here to view the list.

for Recombinant SIRT5 proteinProtein Details

Recombinant human SIRT5 was expressed in E.coli as the full length protein (accession number NM_012241.4) with an N-terminal GST-Tag. The molecular weight of the protein is 65.8 kDa. The purity of the protein is > 70% by SDS-PAGE.

Recombinant SIRT5 SDS-PAGE gel.

Full length Recombinant SIRT5 Protein 12.5% SDS-PAGE, with Coomassie Blue staining.
MW: 65.8 kDa

for Recombinant SIRT5 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SIRT5 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.


Technical Data Sheet

Recombinant SIRT5 protein

Data Thumbnails

Recombinant SIRT5 SDS-PAGE gel.

Recombinant SIRT5 Protein 12.5% SDS-PAGE with Coomassie Blue staining.
MW: 65.8 kDa
(Click image to enlarge and see details).