Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant SIRT6 protein

Aliases: Sirtuin 6, Regulatory Protein SIR2 Homolog, SIR2-Like Protein 6, SIRT-6
Catalog No: 31532 Format: 100 µg $405 Buy Now
Catalog No: 31932 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

Request a quote for a bulk orderBulk Order Button

for Recombinant SIRT6 proteinContents

Full length recombinant SIRT6 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 0.04% Triton X-100, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SIRT6 proteinBackground

SIRT6 (Sir2 homologue 6) is an NAD+ dependant histone deacetylase (HDAC) related to SIRT1 and to the yeast Sir2 protein. SIRT6, originally identified as a tubulin deacetylase, was recently found to be able to deacetylate histone H3 acetylated at lysine 9.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).

for Recombinant SIRT6 proteinApplication Notes

Recombinant SIRT6 protein is useful for histone deacetylase assays and the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Active Motif has a collection of small-molecule epigenetic modulators that target SIRTs - click here to view the list.

for Recombinant SIRT6 proteinProtein Details

Recombinant human SIRT6 was expressed in E. coli as the full length protein (accession number NM_016539) with an N-terminal GST-Tag. The molecular weight of the protein is ~65 kDa. The recombinant protein is > 65% pure by SDS-PAGE.

Recombinant SIRT6 SDS-PAGE gel.

Recombinant SIRT6 protein run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant SIRT6 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SIRT6 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.


Technical Data Sheet

Recombinant SIRT6 protein

Data Thumbnails

Recombinant SIRT6 SDS-PAGE gel.

SIRT6 protein was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.
(Click image to enlarge and see details).