Recombinant DNMT3A (278-432) protein

Aliases: DNA Methyltransferase 3A, DNMT3A2, TBRS, M.HsaIIIA
Catalog No: 31541 Format: 100 µg $410 Buy
Catalog No: 31941 Format: 1 mg $2,900 Buy
Expressed In: E. coli Protein Species: Human

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Recombinant DNMT3A (278-432) was expressed in E. coli cells and is supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.


DNMT3A (DNA methyltransferase 3 alpha) is a de novo methyltransferase. DNMT3A is involved in DNA methylation, in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. DNMT3A has been shown to be important in the regulation of specific patterns of DNA methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and control of gene expression. DNA methylation is generally associated with transcriptional repression.

Trimethylation of Lys36 on histone H3 tail (H3K36me3) is associated with DNA methylation and elongation phase of transcription. PWWP domain of DNMT3A reads this epigenetic mark to guide DNA methylation.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

The peptide corresponding to amino acids 278 - 432 that contains the PWWP sequences of DNMT3A (accession number NP_072046.2) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 43.5 kDa. The recombinant protein is >90% pure by SDS-PAGE.

Recombinant DNMT3A (278-432), GST-Tag gel.

Recombinant DNMT3A (278-432) protein gel.
Recombinant DNMT3A (278-432) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.