Recombinant FTO (ALKBH9) protein

Catalog No: 31572 Format: 20 µg $490 Buy
Catalog No: 31972 Format: 1 mg $3,900 Buy

Request a quote for a bulk order Request Quote

Expressed In: Baculovirus Protein Species: Human


A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.


FTO (Alpha-Ketoglutarate Dependent Dioxygenase, also known as GDFD, ALKBH9, BMIQ14) is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily. FTO specifically demethylates m6A RNA. FTO plays an important role in the regulation of the global metabolic rate, energy expenditure and energy homeostasis. It is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells.

Application Notes

Recombinant FTO protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
3 µM m6A ssDNA oligos (DNA sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3’) were incubated with FTO protein in reaction buffer including 50 mM HEPES pH 7.5, 100 µM 2OG, 100 µM Ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Dot-Blot was used for activity detection.

Protein Details

Recombinant FTO protein was expressed in a Baculovirus expression system as the full length protein (accession number XP_011521615.1) with an N-terminal FLAG-Tag. The molecular weight of FTO is 60.4 kDa.


Recombinant FTO protein, SDS-PAGE gel.
Recombinant FTO protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 60.4 kDa
Purity: > 95%

Recombinant FTO protein activity.
3 µM m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with FTO protein in reaction buffer for 2 hr. at RT. Reaction products spotted onto nylon membrane were detected with m6A antibody (Cat. No. 61495). FTO alone was used as negative control.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.