N6-Methyladenosine (m6A) antibody (pAb)

Catalog No: 61495 Format: 100 µg $435 Buy
Catalog No: 61496 Format: 10 µg $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
Not Applicable
Reactivity:
Other (Not Species Specific)

Applications

Dot Blot Validated

Application Notes


Validated Applications:
DB: 1 - 2 µg/ml dilution

Immunogen

This antibody was raised against 6-Methyladenosine conjugated to KLH.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
N6-Methyladenosine (m6A) antibody (pAb) tested by dot blot analysis.

N6-Methyladenosine (m6A) antibody (pAb) tested by RNA dot blot analysis.
3.3ng of a 22 nt RNA containing four repeats of the GAC consensus for N6-methyladenosine within its sequence was spotted onto a membrane and probed with N6-Methyladenosine antibody (1 µg/ml). The sequence contained various adenosine modifications at all four positions as indicated.
Lane 1: Adenosine.
Lane 2: N6-methyladenosine.
Lane 3: 1-methyladenosine.
Lane 4: 2’-O-methyladenosine.
Lane 5: inosine

N6-Methyladenosine (m6A) antibody (pAb) tested by dot blot analysis.

N6-Methyladenosine (m6A) antibody (pAb) tested by DNA dot blot analysis.
Single-stranded DNA oligonucleotides (amount of oligo in pmoles listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with N-6-methyladenosine antibody (1 µg/ml dilution).
Lane 1: DNA containing a single 6-methyladenosine.
Lane 2: DNA containing unmethylated adenosine.
Lane 3: DNA containing a single 1-methyladenosine.

Background

N6-Methyladenosine (m6A) ) is an RNA modification on the N-6 position of adenosine. This modification has been found to be abundant in the 3’ UTR and stop codons of mammalian mRNA. m6A is associated with miRNA binding sites suggesting a potential role in epigenetic gene regulation. FTO and ALKBH are demethylases for 6-methyladenosine while a multiprotein complex that includes METTL3 functions as the methyltransferase. Recent findings revealed that m6A is also present on metazoan DNA, suggesting a genuine epigenetic role for this modification in the context of DNA as well.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting