A representative Technical Data Sheet (TDS) is provided here. Please refer to your lot-specific TDS upon arrival for specific buffer contents and lot-specific protein concentration.
hnRNPA2B1 (Heterogeneous Nuclear Ribonucleoprotein A2/B1, also known as RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; IBMPFD2; HNRPA2B1) belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. hnRNPA2B1 includes two different isoforms A2 and B1. It is reported that hnRNPA2B1 is a nuclear ‘‘reader’’ of the m6A mark, acting as an adaptor that recruits the Microprocessor complex to a subset of precursor miRNAs, facilitating their processing into mature miRNAs.
Recombinant hnRNPA2B1-B1 protein is suitable for use in evaluation of protein-protein interactions and RNA binding assays.
Activity Assay Details: 75 pmol biotinylated m6A ssDNA oligos (5’-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3’) were incubated with hnRNPA2B1 protein in binding buffer including 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 3 hr at 4%deg;C. The DNA-hnRNPA2B1 compounds were incubated with streptavidin beads for 1 hr at 4C and pull-down complexes were run on SDS-PAGE gel, stained with Coomassie Blue. Biotinylated, unmethylated ssDNA oligos used as a negative control.
Recombinant hnRNPA2B1-B1 protein is the B1 isoform of hnRNPA2B1 and was expressed in a Baculovirus expression system as the full length protein (accession number NP_112533.1) with an N-terminal FLAG-Tag. The molecular weight of hnRNPA2B1-B1 is 38.4 kDa.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.