Rad51 antibody (pAb)

RRID: AB_2614951
Aliases: RECA, BRCC5, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930
Catalog No: 39194 Format: 200 µg $445 Buy
Catalog No: 39195 Format: 10 µg $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
37 kDa
Reactivity:
Human

Applications

Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1 - 5 µg/ml dilution

Published Applications: see references

Immunogen

This Rad51 antibody was raised against full-length human Rad51 expressed in E. coli.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

Rad51 pAb tested by immunofluorescence.
HEK293 cells stained with Rad51 polyclonal antibody (green) and DAPI (blue).

Background

Rad51 (BRCC5, RecA) is known to be involved in the homologous recombination and repair of DNA. Rad51 can interact with the ssDNA-binding protein RPA and Rad52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Rad51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad51 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting